Sunday 6 April 2014

Gram positive staining vs Gram negative staining


Gram positive staining
Gram negative staining
Gram-positive are those that are stained dark blue or violet by Gram staining.
This is in contrast to Gram-negative, which cannot retain the crystal violet stain, instead taking up the counterstain (safranin or fuchsine) and appearing red or pink
Gram-positive organisms are able to retain the crystal violet stain because of the high amount of peptidoglycan in the cell wall. 
Gram-positive cell walls typically lack the outer membrane found in Gram-negative bacteria.
Positive stain stick with specimen and gives it's color


Negative dye doesn't stick with the specimen but settle around it's outer boundary and forming a silhouette. that negative stain produce a dark back ground around the cell


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